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KMID : 0381120140360050565
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Genes and Genomics
2014 Volume.36 No. 5 p.565 ~ p.572
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Molecular cloning, characterisation and expression of the translationally controlled tumor protein gene in rock bream (Oplegnathus fasciatus)
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Chae Yeong-Sun
Lee Jung-Ho
Shim Won-Joon
Jung Jee-Hyun
Kim Ju-Won
Park Chan-Il
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Abstract
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Translationally controlled tumor protein (TCTP) is an important immune regulator that has been implicated in a number of cellular processes, including cell growth, cell cycle progression, apoptosis regulation and protection of cells against various environmental stresses. In this study, we cloned and characterised TCTP from rock bream (Oplegnathus fasciatus), which is an economically important species in the Korean aquaculture industry. The full-length rock bream TCTP (RbTCTP) cDNA was of 1,041 bp and contained an open reading frame of 513 bp, which encoded 170 amino acids. The 5′ untranslated region (UTR) was 90 bp, while the 3′ UTR was 438 bp, containing a polyadenylation signal. RbTCTP showed 76, 75 and 74 % amino acid sequence identities to those of tilapia (Oreochromis niloticus), orange-spotted grouper (Epinephelus coioides) and Japanese sea perch (Lateolabrax japonicus), respectively. The positions of microtubule binding region, Ca+ binding region and TCTP signature regions in RbTCTP were similar to other fish species and mammals. RbTCTP mRNA expression level was highest in the gill compared to other tissues. The level of RbTCTP mRNA expression was significantly regulated by injection of red seabream iridovirus, Streptococcus iniae and Edwardsiella tarda.
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KEYWORD
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Rock bream, Translationally controlled tumor protein, Cloning, Expression analysis
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